Verifying the TiPED Methodology:

A whimsical visual style may appeal to some players, while others will care more about the speed of the spins. Someone considering Huff N’ More Puff might begin by checking how the controls are explained. There is no substitute for checking the conditions that apply to each round.

Use of TiPED* to detect endocrine disrupting activity of six known EDCs: BPA, Atrazine, Perfluorinated Compunds, Phthalates, Organotins and Perchlorate

 

BPA

Atrazine

Perfluorinated compounds

Phthalates

Organotins

Perchlorate
Tier/Assay Type

Specific Assay
Computer-Based
Chemical reactivity
Physio-chemical properties

[151]
Docking modeling

[152]

[153]

[154]
QSAR

[155]

[156]
Targeted Cell Assays
Tox21 qHTS

[157]

[157]

[157]
Cell Processes Assays
MCF7 cell prolifera-tion assay

[158]

[159]

[160]
Prostate cancer cell prolifera-tion assay, PSA assay

[161]

[162]
3T3-L1 adipogen-esis assay

[163]

[163]

[89]
GH3 T-screen assay

[164]

[165]
Fish/Amphibia
Zebrafish rapid develop-mental toxicity HTS

[107, 108]

[109]

[110, 111]

[40]
Aquatic EDC reporter assays

[42, 166, 167] (ER)

[43] (TH)
Medaka and fathead minnow reproduc-tive assays

[168]

[169]

[170]

[171]

[172]
Xenopus metamorphosis assay

[173]

[174]

[175]
Xenopus sexual dimorph-ism assays

[176]

[177]

[178]

[179]
Frog metamor-phosis assay

[173]

[174]

[175]
H. argus color change assay
Xenopus corticoid assay

[51]
Mammalian
Asthma

[180]
Brain sexual dimorph-ism assay

[129]

[181]

[182]
Mammary carcino-genesis assay

[138]

[183]
Mammary gland morpholo-gy assay

[64]

[184]

[185, 186]

[187]
Maternal behavior assay

[139]

[188]
Obesity / Metabolic syndrome assays

[189, 190]

[191]

[192]

[89]
Prostate carcinogenesis assay

[193]
Sexual dimorph-ism behavior assays

[129]
Tissue gene expres-sion assay

[194]

[195]

[196]

[197]

[198]

[199]

*Based on a search of the peer-reviewed scientific literature.