|Corticosteroid secretion||Oncorhynchus mykiss||Corticosteroid secretion in response to ACTH|||
|Rapid developmental toxicity HTS (in Tier 2)||Zebrafish||Morphological endpoints (edema, bent body axes, pigmentation anomalies, and organ malformations)||[40, 107-111]|
|Fish sex development test||Fathead minnow
|Designed to detect (anti-) estrogenic and (anti-) androgenic effects. Animals are exposed to test chemical before the onset of sexual differentiation.
Vitellogenin induction in males/inhibition in females.
Development of intersex
|Fish Two Generation Assay||Fathead minnow
|Whole body, serum, tissue T4 levels|||
|Locomotion medium throughput assay (in Tier 2)||Zebrafish||Can identify subtle developmental abnormalities between the nervous and musculoskeletal systems|||
|Sex specific behavior||Zebrafish||Sex specific behaviors (aggressive: nipping, chasing, circling, avoiding, and reproductive: female association, spawning, chasing, and nipping)|||
|Short-term reproduction assay/ 21-day fish assay||Fathead minnow
|Designed to detect (anti-) estrogenic and (anti-) androgenic effects. Mature male and female fish will be monitored during a 21-day chemical exposure; survival, reproductive behavior, and secondary sexual characteristics will be observed while fecundity and fertilization success will be monitored daily. At termination of the assay, measurements will be made of a number of endpoints reflective of the status of the reproductive endocrine system, including the GSI, gonadal histology, and plasma concentrations of vitellogenin.||, ;|
|Transgenic reporter lines||Zebrafish||Current lines can detect estrogenic activity and aromatase induction. More transgenic lines are being developed.|
|Corticosteroid secretion||X. laevis
|Corticosteroid secretion in response to ACTH|||